FORMATION OF DNA COMPLEXES WITH SPERMINE AND POLYLYSINE DERIVATIVES
Abstract
The interactions between DNA and positively-charged molecules (i.e. surfactants and polymers) have been studied in aqueous solutions through Dynamic (DLS) and Static (SLS) Light Scatering techniques. Contrary to the common believe about polyelectrolyte complexes, it was determined that the DNA complexes prepared in this studied contained only one polyanion molecule. When complexes are formed with polylysine-b-polyethylene glycol block copolymer in aqueous solution, 100% of the DNA charges are neutralized with a small excess of the cation.Downloads
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